DTIC ADA221157: Toxin Induced Activation of Rat pdf

DTIC ADA221157: Toxin Induced Activation of Rat

More Book Details

Description of the Book:

The effects of microcystin-LR, a trichothecene, T-2 and saxitoxin on membrane lipid mediators of inflammatory processes were evaluated in cultured rat hepatocytes. Microcystin-LR significantly stimulated the release of prostacyclin, measured as 6-keto PGF(a a) by 38% (p0.01) and thromboxane B2 (TxB2) by 50%, (p0.001) in a concentration-dependent manner. The trichothecene toxin, T-2 enhanced the release of prostaglandin F(2a) (PGF2a) by 24% (p 0.05) and arachidonic acid by 29% (p 0.05); while saxitoxin failed to cause the release of prostaglandins or arachidonic acid. Incorporation of arachidonic acid into the lipid pool was reduced to 47% (p 0.025) by 1 micromole microcystin-LR. Changes in phospholipid classes indicated that prostaglandin formation induced by microcystin-LR was due to the release of arachidonic acid from the phosphatidylinositol pool. No statistically significant effect of toxin was observed on other classes of phospholipids or neutral lipids. A 10% increase in phosphatidylcholine in hepatocytes treated with microcystin-LR may have resulted from conversion of phosphatidylethanolamine to phosphatidylcholine via the N- methylation pathway.

These results indicate that microcystin-LR has important effects on the regulation of inflammatory mediator synthesis in hepatocytes

• Creator/s: Defense Technical Information Center
• Date: 4/25/1990
• Year: 1990
• Book Topics/Themes: DTIC Archive, Naseem, Syed M, ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD, *LIVER, *CELLS(BIOLOGY), *TOXINS AND ANTITOXINS, *PROSTAGLANDIN, RATS, SYNTHESIS, NEUTRAL, LIPIDS, METABOLISM, RELEASE, INFLAMMATION, FATTY ACIDS, PHOSPHOLIPIDS

An excerpt captured from the PDF book