DTIC ADA349126: The Regulation of Gene Expression pdf

DTIC ADA349126: The Regulation of Gene Expression

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We have characterized genes encoding heat shock proteins and proteins of the ubiquitin system. Constitutive forms of the HSP60 and 70 families of heat shock proteins and an inducible form of HSP60 occur in symbiotic and aposymbiotic Aiptasia pallida. We have constructed cDNA libraries from aposymbiotic A. pallida and symbiotic A. pallida and Anthopleura eligantissima. We have a complete sequence for A. pallida HSP60. We have completed the comparison of protein profiles of A. eligantissima (see J. Exp Biol. 1996, 199:883-892) and have identified three symbiosis-specific proteins. One is carbonic anhydrase with an apparent molecular weight of 31 kDa and a pI of 6.3. We have obtained N-terminal sequence for second protein; it has an apparent molecular weight of 32 kDa and a pI of 7,9. It does not align with any sequences in the GenBank data base. A third symbiosis-specific protein, having an apparent weight of 30 kD and a pI of 5.6, crossreacts with a monoclonal anti-HSP70 antibody.

The synthesis of this protein is enhanced in animals subjected to elevated temperatures and in animals maintained in the dark. Further investigations of these proteins are underway by Dr. Virginia Weis at Oregon State University

• Creator/s: Defense Technical Information Center
• Date: 7/13/1998
• Year: 1998
• Book Topics/Themes: DTIC Archive, Levine, R. P., STANFORD UNIV PACIFIC GROVE CA HOPKINSMARINE STATION, *GENES, *SHOCK(PATHOLOGY), *HEAT STRESS(PHYSIOLOGY), DATA BASES, CONTROL, VIRGINIA, SYNTHESIS, HIGH TEMPERATURE, PROTEINS, COMPARISON, MOLECULAR WEIGHT, PROFILES, ANIMALS, CARBONIC ANHYDRASE

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