DTIC ADA453904: Development and Testing of a pdf

DTIC ADA453904: Development and Testing of a

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This paper describes a novel simulant test particle that allows for the calibration of biodetection systems without causing safety concerns. Specifically, this test particle is a polystyrene sphere, of an aerodynamic diameter similar to that of Bacillus anthracis, with Bacillus globigii genomic DNA attached to the surface. The DNA attachment is strong enough to withstand aerosolization and collection by a SpinCon sampler, and yet weak enough to detach during the GeneXpert analysis. Tests performed at MRI have shown that the tagged beads can be collected in the SpinCon and then successfully analyzed in the GeneXpert without modifying currently established analysis protocols

• Creator/s: Defense Technical Information Center
• Date: 11/19/2003
• Year: 2003
• Book Topics/Themes: DTIC Archive, Brown, Kelly L, MIDWEST RESEARCH INST KANSAS CITY MO, *TEST AND EVALUATION, *BIOLOGICAL AGENT DETECTORS, SYMPOSIA, POLYSTYRENE, BACILLUS SUBTILIS, MICROSPHERES, DEOXYRIBONUCLEIC ACIDS, CALIBRATION, BACILLUS ANTHRACIS

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