DTIC ADB258646: A New Insulin Like Growth pdf

DTIC ADB258646: A New Insulin Like Growth

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Mac25 is the first member of the IGFBP-rP family (IGFBP-rP1). IGFBP-rP1 is expressed in normal human mammary epithelial cells (HMECs) and upregulated in senescent HMECs. IGFBP-rP1 is not expressed in twelve/sixteen breast cancer cell lines and its expression is absent in estrogen receptor positive (ER+) breast cancer cell lines. IGFBP-rP1 may regulate HMEC growth. We tested the hypothesis that it suppresses growth in ER+ breast cancer cells by transducing the cDNA into normal and human breast tumor cultures. We are testing the function of IGFBP-rP1 in normal HMECs by transducing AG11132 normal cells. We will determine if IGFBP-rP1-rP1 is regulated at the transcriptional level by characterization of the promoter. We are determining the binding properties of IGFBP-rP1 by growth assays in presence of IGF-I, IGF-II, R3-IGF-I, and R6-IGF-II and immunoprecipitation/phosphatase assays. We have determined IGFBP-rP1 tissue specificity by mRNA in situ hybridization

• Creator/s: Defense Technical Information Center
• Date: 9/1/1999
• Year: 1999
• Book Topics/Themes: DTIC Archive, Swisshelm, Karen, WASHINGTON UNIV SEATTLE, *PROTEINS, *BREAST CANCER, EPITHELIUM, CLONES, CHEMICAL PRECIPITATION, CELLS(BIOLOGY), IMMUNOASSAY, INSULIN, HYBRIDIZATION, PHOSPHATASES, ESTROGENS

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